AzuraQuant Probe 1-Step qPCR Mix NoRox 500 Reactions / 5 x 1 mL

AzuraQuant Probe 1-Step qPCR Mix NoRox is a ready-to-use 2x master mix and companion 20x thermostable reverse transcriptase for use in highly sensitive RT-qPCR and has been formulated for probe-detection technology, including TaqMan, Scorpions and molecular beacon probes. AzuraQuant Probe 1-Step qPCR Mix NoRox is powered by Azura HS Taq DNA Polymerase, AzuraSprint Reverse Transcriptase, and an optimized buffer chemistry, providing robust first-strand cDNA synthesis and real-time PCR in a single tube. The Mix delivers earlier quantification cycle values (Ct) and broad range detection for increased sensitivity, speed, reliability and reproducibility. AzuraQuant Probe 1-Step qPCR Mix NoRox can be used to quantify a specific target RNA from either total RNA or mRNA while reducing the number of pipetting steps and time to result. The AzuraQuant Probe 1-Step qPCR Mix NoRox requires little if any optimization and employs rapid antibody-mediated hot-start activation and a processive enzyme chemistry, providing unmatched performance in muliplexing.

• Applications: Gene expression analysis directly from RNA; Low-copy number detection from viral DNA samples; gene expression analysis; pathogen detection; detection of sequence variants
• Flexibility: Compatible with Standard and fast cycling instruments and a wide range of cycling parameters
• Accurate Quantification: Hot-start chemistry reduces primer-dimer formation and allows room-temperature assembly
• Superior Multiplex Capability: advanced formulation provides unrivaled sensitivity in singleplex as well as complex multiplex assays